Overview:  The Zeiss Lightsheet 7 microscope is designed for fast, gentle 3D/4D imaging of samples labeled with fluorescent probes.  Both live and fixed samples can be imaged. Samples are lowered from above into an imaging chamber containing media of the appropriate refractive index.  It is located in BPB G05.  Rate information is available here.

Lasers:

• 405, 488, 561, 638 nm

Imaging Optics:

• 5X 0.16NA (for live samples in aqueous media)
• 5X 0.16 NA (for fixed samples, correction collar adjustable for different refractive index media up to RI=1.53)
• 10X NA 0.5 (for live samples in aqueous media)
• 20X NA 1.0 (for live samples in aqueous media)
• 20X NA 1.0 (for fixed samples, correction collar adjustable for different refractive index media up to RI=1.45)

Illumination Optics (2 each for forming the lightsheet):

• 5X NA 0.1
• 10X NA 0.2

Imaging Chambers:

• Live chamber for samples in aqueous media
• Fixed sample chamber (small)
• Fixed sample chamber (large, 28 x 31 x 49 mm)

Detectors:

• 2 PCO edge 4.2 sCMOS cameras for dual channel imaging
  • 2048 x 2048 pixel resolution
  • 100 fps maximum frame rate
  • up to 82 % quantum efficiency

Software:

• Zen Black for acquisition
• Zen Blue for analysis
• Arivis for large volume 3D rendering and analysis (on separate analysis PC)