Overview: The Nikon AXR is a point scanning confocal built around the inverted Ti2E microscope.  The large 25 mm field of view and resonant scanner are optimized for rapid imaging of samples.  The DUX-VB confocal detector features 4 sensitive GaAsP detectors that can be flexibly configured for a range of dyes via spectral detection.  6 laser lines provides a range of excitation options for a variety of fluorescent dyes.  In addition to confocal microscopy, the system has an iLAS2 illumination module for TIRF imaging.  This device can perform simultaneous TIRF imaging and photostimulation. TIRF, widefield, and transmitted light images are collected on a Photometrics Prime 95B sCMOS camera.  The instrument is located in the Biology and Physics Building (BPB), room G05D.  Click here for rate information.

 

 

 

 

 

 

 

 

Specifications

Microscope: 

• Inverted microscope Nikon Ti2E
• Motorized x/y stage
• PerfectFocus optical z drift correction
• MCL Nano-Z200 piezo z stage

Laser Unit for Confocal:

• LUA-S6 405/445/488/514/561/640

Laser Unit for TIRF:

• LUNF XL 405/488/561/640

TIRF/Widefield Camera

• Photometrics Prime95B

Objective Lenses:

• 10X PLAN APOCHROMAT LAMBDA D, DIC
• 20X PLAN APOCHROMAT LAMBDA D, DIC
• 40X/1.30 PLAN FLUOR, Oil, DIC
• 60X/1.40 PLAN APOCHROMAT LAMBDA D, Oil, DIC
• 60X/1.20 PLAN APO, WI, DIC
• 100X/1.49 APO TIRF, Oil, DIC

Software:

• NIS-Elements C Confocal Package, JOBS for Scanning Plates and Creating Custom Acquisition and Analysis Routines, NIS-Elements 2D/3D, Deconvolution Suite, NIS.ai Artificial Intelligence Module, General Analysis 2 and 3