Overview: STORM and PALM are localization super resolution methods that provide up to a 10-fold improvement in resolution compared to conventional fluorescence microscopy. These approaches temporally separate the emission of individual fluorophores in a sample with specialized imaging conditions. “Localizations” are accumulated over time to build the final image. Each molecules’ recorded fluorescence distribution is fit to a Gaussian function in post-processing to identify its position. The aggregated coordinates are displayed as a reconstruction.
This is an add-on modality to the Andor Spinning Disk/Widefield/TIRF system that can provide down to 20 nm lateral resolution in one or two channels. There are a number of sample considerations for STORM/PALM imaging. Please consult our sample prep guide. The system is located in the Biology and Physics Building (BPB), room G05D. Click here for rate information.
STORM Modality Specifications:
- Inverted Stand Nikon Eclipse TiE
- ASI Motorized x-y stage
- ASI piezo z stage insert
- 405 nm photoactivation/conversion laser
- 561 nm imaging laser
- 633 nm imaging laser
- 100X/1.49 Plan Apo oil, DIC
- Andor iXon DU-897 back-illuminated frame transfer EMCCD
- Micro-Manager for acquisition
- ThunderSTORM ImageJ plugin for analysis